Oncology Central

The thrills and agonies of using next-generation sequencing for somatic mutation detection in cancer

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In the 1990s, the field of molecular biology was catapulted into the diagnostic arena as the PCR technology offered performance characteristics and turnaround times more suitable for a clinical laboratory setting than all previous molecular techniques. Over the next 10 years, limitations of the PCR were quickly overcome by the introduction of real-time PCR chemistries and instruments that automated amplification and detection, as well as sample extraction in some turnkey platforms [1].

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