Original Publication Date: 8 June, 2014
Publication / Source: Nanomedicine
Authors: Saheel Bhana, Elise Chaffin, Yongmei Wang, Sanjay R Mishra & Xiaohua Huang
Aim: To develop a simple assay for the capture and detection of rare cancer cells in whole blood using iron oxide–gold (IO–Au) nanoparticles.
Materials & methods: IO–Au nanoovals (NOVs) were synthesized, coated with Raman tags and linked with antibodies targeting breast cancer. An integrated system was constructed for on-line magnetic cell capture and surface-enhanced Raman scattering (SERS) detection. The capabilities of IO–Au SERS NOVs to capture and detect rare cancer cells in blood were investigated in the integrated system using circulating tumor cell-mimic SK-BR-3 cells.