Original Publication Date: 4 April, 2014
Publication / Source: Nanomedicine
Authors: Stephan Hackenberg, Agmal Scherzed, Antje Gohla, Antje Technau, Katrin Froelich, Christian Ginzkey, Christian Koehler, Marc Burghartz, Rudolf Hagen & Norbert Kleinsasser
Aim: To characterize molecular mechanisms underlying photocatalytic cell death of head and neck squamous cell carcinoma (HNSCC) by zinc oxide nanoparticles (ZnO-NPs). Materials & methods: Human HNSCC-derived FaDu cells were incubated with ZnO-NPs followed by UVA-1 irradiation. Cytotoxicity was assessed by MTT assay and annexin-V propidium iodide test. Autophagy was detected by autophagosome accumulation, conversion of light chain 3 I to II, and lysosomal activity. The generation of reactive oxygen species was measured using the 2´,7´-dichlorofluorescein-diacetate test.